Metabolism in preimplantation mouse embryos
نویسندگان
چکیده
منابع مشابه
Heat shock memory in preimplantation mouse embryos.
To investigate the consequences of possible physiologic stress to embryos caused by in vitro fertilization procedures, we used heat shock response in preimplantation mouse embryos as a model. A heat shock "memory" was discovered that renders cleavage-stage embryos more responsive at the transcriptional level to secondary perturbation with very low doses of heat, even several cell cycles after t...
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The role of microtubules during compaction of the 8-cell-stage mouse embryo was investigated using the drugs Taxol (which leads to a non-controlled polymerization of tubulin) and Nocodazole (which causes depolymerization of microtubules). Taxol inhibits compaction in most non-compacted embryos and reverses it in already compacted embryos. These effects were observed on both cell flattening (as ...
متن کاملCell membrane regions in preimplantation mouse embryos.
Cell membrane regions characterized by alkaline phosphatase activity are described in cleaving mouse embryos and early blastocysts. Enzyme activity is demonstrated by light and electron microscopy, from the late 4-cell stage onwards, on the cell surfaces between blastomeres but not on the outer surface of the embryo. Experiments with dissociated morulae show that this is probably not an artifac...
متن کاملThe Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos
Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...
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Background: In vitro culture of mammalian embryos can slow or stop growth completely. This may be due to the medium used, pH, temperature, or light. There is considerable concern about the harmful effect of light in the laboratory environment. Cell number and apoptosis are useful parameters that indicate embryonic development and health. In this study, we assessed these two factors in the blas...
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ژورنال
عنوان ژورنال: Journal of Biosciences
سال: 1984
ISSN: 0250-5991,0973-7138
DOI: 10.1007/bf02716714